Main Article Content
The physico-chemical properties of the causal agent of virus disease of Coccinia barteri (Hook. f.) Keay were studied. The virus causing the disease was characterized using diagnostic tools such as host range, longevity in vitro, thermal inactivation point, dilution endpoint and aphid transmission. The virus was mechanically transmitted from the natural host (C. barteri) to the healthy test plants in the green house. In the biological properties, the virus was successfully transmitted by Aphis spiraecola (obtained from Chromolaena odorata (L.) R. M. King & H. Rob.) from infected Cucumeropsis mannii Naudin to a healthy C. mannii in a non-persistent manner and had a narrow host range limited to the family Cucurbitaceae. In the physico-chemical properties based on crude sap with an unknown virus concentration, beyond which infectivity was lost. It was readily inactivated by heating to 35 – 65°C for 10 minutes in determination of thermal inactivation point. The virus had a longevity in vitro of between 4 – 5 days beyond which it was non-infectious. Symptoms induced by the virus were leaf cupping, mottle chlorosis, blisters, stunted growth, rugosity, leaf malformation and mosaic patterns.
Matthews RE. Plant virology (3rd ed) New York, Academic Press. 1991;234.
Hull R. Plant virology. 5th Edn. Cambridge, MA: Academic Press. 2014;323.
Wren J, Roossinck M, Nelson R, Scheets K, Palmer M, Melcher U. Plant virus biodiversity and ecology. PLoS Biol. 2006;4:80.
Owens R, Flores R, Di Serio F, Li S, Pallas V, Randles J. Virus taxonomy: Ninth report of the international committee on taxonomy of viruses. Amsterdam: Elsevier. 2012;23.
Min B, Feldman T, Ali A, Wiley G, Muthukumar V, Roe B. Molecular characterization, ecology, and epidemiology of a novel Tymovirus in Asclepias viridis from Oklahoma. Phytopathology. 2012;102:166–176.
Shates T, Sun P, Malmstrom C, Dominguez C, Mauck K. Addressing research needs in the field of plant virus ecology by defining knowledge gaps and developing wild dicot study systems. Frontier Microbiology. 2019;9:33-35.
Xu P, Chen F, Mannas J, Feldman T, Sumner L, Roossinck M. Virus infection improves drought tolerance. New Phytol. 2008;180:34–38.
Roossinck MJ. The good viruses: Viral mutualistic symbioses. Natural Review of Microbiology. 2011;9:99–108.
Roossinck MJ. Plant virus ecology. PLoS Pathog. 2013;9:100-304.
Westwood J, McCann L, Naish M, Dixon H, Murphy A, Stancombe M. A viral RNA silencing suppressor interferes with abscisic acid-mediated signalling and induces drought tolerance in Arabidopsis thaliana. Molecular Plant Pathology. 2013;14:158–170.
Holstein N, Renner S. Coccinia (Cucurbitaceae) gains two new species from East Africa, three new synonyms and one new combination. Kew Bull. 2010;65: 435–441.
Holstein N, Renner S. A dated phylogeny and collection records reveal repeated biome shifts in the African genus Coccinia (Cucurbitaceae). BMC Evolutionary Biology. 2011;11:28.
Owolabi A, Rabenstein F, Ehrig F, Maiss E, Vetten H. Strains of Moroccan watermelon mosaic virus isolated from Lageneria breviflorus and Coccinia barteri in Calabar, Southeastern Nigeria. International Journal of Virology. 2012;8: 258–270.
Chukwuma EC, Soladoye MO, Feyisola RT. Traditional medicine and the future of medicinal plants in Nigeria. Journal of Medicinal Plants Studies. 2015;25:43–48.
Dijkstra J, De Jager CP. Practical plant pathology, New York; Springer Berlin Heidelberg. 1998;100–107.
Webb RE, Scott HA. Isolation and identification of Water melon mosaic viruses. Phytopathology. 1965;52:12–21.
Owolabi AT, Ekpiken EE. Transmission efficiency of two strains of moroccan watermelon mosaic virus by two clones of Aphis spiraecola (Patch). International Journal of Virology. 2014;10:253–262.