International Journal of Pathogen Research

Antifungal Potential of Acetone and Ethyl Acetate Extracts of Thevetia peruviana on Development of Phytophthora colocasiae, Causal Agent of Late Blight of Taro (Colocasia esculenta (L.) Schott) from Three Agro-Ecological Zones of Cameroon

International Journal of Pathogen Research, Volume 11, Issue 3-4, Page 1-12
DOI: 10.9734/ijpr/2022/v11i2208

Abstract


Aims: This study was aimed to evaluate the antifungal activities of acetone and ethyl acetate extracts of Thevetia peruviana seeds on the in vitro growth of the fungus.

Study Design: A randomized sample block design containing four treatments (T- = Negative control; T2= Ethyl acetate extract; T3= Acetone extract; T+=Callomil Plus) with three repetitions was used. Plant extracts were used at three concentrations: C1: 12.5 µl/ml; C2: 25 µl/ml and C3: 50 µl/ml; the chemical fungicide at the dose of 12.5 μL/ml.

Place and Duration of Study: The study was conducted in the University of Yaoundé 1, Faculty of Sciences, Department of Plant Biology, Laboratory of Phytopathology and Crop Protection, and in the Institute of Agricultural Research for Development (IARD) of Yaoundé, Laboratory of Phytopathology, during the year 2019-2020.

Methodology: acetone and ethyl acetate extracts of T. peruviana were prepared and used at concentrations of 12.5, 25 and 50 µl/ml. P. colocasiae was isolated from infected taro leaf cultivars "Macumba or Ibo coco" located in three different regions: west, Littoral and Centre. The various explants were were put in V8 agar medium and maintained in pure culture.  Mycelial fragments of P. colocasiae of about 0.8 cm in diameter were cut and placed in sterile Petri dishes containing Potato Dextrose Agar (PDA) medium supplemented with different concentrations of plant extracts and incubated at 23±1°C for seven days for the evaluation of the radial growth.

Results: The results obtained showed that the acetone and ethyl acetate extracts have completely inhibited the growth of the strain of West at 25 μ/ml while total inhibition of the pathogen was not obtained with strain of Centre region. The lowest inhibition was obtained with the strain of Littoral region: 93.88 % for acetone extract and 90.78 % for ethyl acetate extract compare to 100 % for west and Centre region at highest concentration.

Conclusion: The acetone and ethyl acetate extracts at the concentration of 25 μ/ml totally inhibited the in vitro radial growth of some strains of P. colocasiae. These extracts, which are effective against P. colocasiae, may substitute fungicides in the fight against taro leaf blight.

Keywords:
  • Extracts of Thevetia peruviana
  • antifungal potential
  • Phytophthora colocasiae
  • taro

How to Cite

Essomé, C. S., Djuissi, D. T., Thierry, M., Mvondo, D. N., Kuaté, W. N. T., Ngatsi, P. Z., Bolie, H., Nsangou, A. K., Heu, A., Mboussi, S. B., & Ambang, Z. (2022). Antifungal Potential of Acetone and Ethyl Acetate Extracts of Thevetia peruviana on Development of Phytophthora colocasiae, Causal Agent of Late Blight of Taro (Colocasia esculenta (L.) Schott) from Three Agro-Ecological Zones of Cameroon. International Journal of Pathogen Research, 11(3-4), 1–12. https://doi.org/10.9734/ijpr/2022/v11i2208

References

FAOSTAT. FAO economic and social department. The statistics division. Major Food and Agricultural Commodities and Producers. 2018.

Available:http://faostat.fao.org/default.aspxAeccess:4th February 2020.

Plantvillage. Cocoyam; 2018.

Aeccess : June 8, 2020.

Available:https://www.plantvillage.org/en/topics/cocoyam

Charles ES, Jules Patrice ND, Alain H, William KT, Dorothée MN, Patrice NZ, Godwill C, Zachee A. Efficacy of methanolic and aqueous extracts of Thevetia peruviana (pers.) K. Schum on growth of Phytophthora colocasiae Racib, causal agent of taro late blight in Cameroon. Journal of Applied Life Sciences International. 2021;24(4):44-54.

Guarion L. Taro leaf blight in Cameroon. Agricultural Biodiversity Weblog.

Available:http://agro.Biodiver.se/2010/07/ taro-leaf-blight-in-Cameroon/

(Accessed on 15 May 2020).

Asseng CC, Ebongo LE, Nanda DGL, Akono NP, Mbida JA, Ngono NA, Ambang Z, Monkam TF, Djouokep LG. Study of antagonistic beneficial microorganisms to Phytophtora colocasiae, causal agent of taro mildew (Colocasia esculenta (L.) Schott). Plant. 2017;5(3):51-60.

Adinde JO, Anieke UJ, Nwankwo OG, Agu CJ, Aniakor AC, Nwagboso AA, Eze CO. Incidence and severity of taro leaf-blight in Iwollo, South-Eastern Nigeria. Int. J. Curr. Res. Biosci. Plant Biol. 2016;3(10):163168.

Tsopmbeng GR, Lienou JA, Megaptche CJP, Fontem DA. Effet of pH and temperature levels on In vitro growth and sporulation of Phytophthora colocasiae, taro leaf blight pathogen. In. J. Agro. Agri. R. 2014;4(4):202-206.

Cabi. Phytophthora colocasiae (taro leaf blight).

Available:http://www.cabi.org/isc/datasheet /40955

Mishra AK, Sharma K, Misra RS. Effect of benzyl amino purine on the pathogen growth and disease development of taro leaf blight caused by Phytophthora colocasiae. J. Pl. Pathol. 2008;90(2): 191-196.

Elvina P, Forrest S, Emperatriz PD. Caracterization of some properties of starches isolated from Xanthosoma sagittifolium (tannia) and Colocasia esculenta (taro). Carbohyd. Polym. 2005; 60(2):139-145.

Hassan S, Dubey VK, Bhagat KP. Effect of insecticides and plant products against shoot and fruit borer of okra, Earias vittella (Fab.). Agric. Sci. Digest. 1998;18(2):120-122.

Jesus WC, Vale FX, Coelho RR, Haub Zambolin L, Costa LC, Bergamin FB. Effects of angular leaf spot and rust on yield loss of Phaseaolus vulgaris L. Phythopathology. 2001;91:1045-1053.

Ambang Z, Ndongo B, Amayana D, Djilé B, Ngoh JP, Chewachong GM. Combined effect of host plant resistance and insecticide application on the development of cowpea viral diseases. Austr. J. Crp. Sc. 2009;3(3):167-172.

Pohe J, Agneron TA. L’huile des graines de neem, un fongicide alternatif à l’oxyde de cuivre dans la lutte contre la pourriture brune des cabosses de cacaoyer en Côte d’Ivoire. J. Appl. Biosci. 2013;62:4644-4652.

DOI: 10.4314/jab.v62i0.86147

Ngassoum BM, Ngamo LS, Goudoum A. Protection post-récolte du mais par des insecticides peu rémanents: les huiles essentielles. In: Kapseu C, Nganhou J, Boudrant J, Crouzet J. (eds). Séchage et technologie post-récolte. Cameroun. 2002; 240-246.

Djeugap FJ, Fontem DA, Tapondjou AL. Efficacité In vitro et in vivo des extraits de plantes contre le mildiou (Phytophthora infestans) de la morelle noire. Int. J. Biol. Chm. Sci. 2011;5(6):2205-2213.

DOI: 10.4314/ijbcs.v5i6

Makun HA, Anjorin ST, Adeniran LA, Onakpa MM, Muhammad HL, Obu OR. Toxic constituents of different provenances of Jatropha curcas and Ricinus cumunis seeds on Fusarium verticilliodes and Aspergillus flavus in yam. J. Agric. Biol. Sci. 2011;6(6):22-27.

Abdel-Rahman T, Hussein AS, Beshir S, Hamed AR, Ali E, El-Tanany SS. Antimicrobial activity of terpenoids extracted from Annona muricata seeds and its endophytic Aspergillus niger strain SH3 either singly or in combination. Open Access Maced. J. Med. Sci. 2019;7(19): 3127-3131.

DOI: 10.3889/oamjms.2019.793

Ambang Z, Ngoh Dooh J.P, Essono G, Bekolo N, Chewachong G, Asseng CC. Effect of Thevetia peruviana seeds extracts on in vitro growth of four strains of Phytophthora megakarya. Plant Omics Journal. 2010;3(3):70-76.

Mboussi SB, Ambang Z, Ndogho P, Ngoh Dooh JP, Manga Essouma F. In vitro antifungal potential of aqueous seeds extracts of Azadirachta indica and Thevetia peruviana against Phytophthora megakarya in Cameroon. J. Appl. Life Sci. Int. 2016;4(4):1-12.

Essomé SC, Ngoh Dooh JP, Heu A, Ndogho PA, Ngatsi ZP, Chewachong G, Ambang Z. Évaluation des activités antifongiques des extraits de graines de Thevetia peruviana contre Phytophthora colocasiae (Oomycètes) agent causal du mildiou du taro (Colocasia esculenta (L.) Schott. J. Appl. Biosci. 2020;151:15584-15597.

DOI: 10.35759/JABs.151.7

Ngatsi ZP, Bekolo N, Yanga MNM, Tize Tize, Azafack NS, Daouda K, Kuate TNW, Djiéto-Lordon L. Effect of extracts from seeds of Thevetia peruviana (Pers.) K. Schum against cassava root scale Stictococcus vayssierei Richard (Hemiptera: Stictococcidae) in field. Int. J. Biosci. 2020;16(3):536-547.

DOI: 10.12692/ijb/16.3.536-547

Le Ven J. Contribution à l’étude du lien entre Annonaceae et parkinsonismes: identification et quantification d’acétogénines par déréplication; métabolisation de phase I et approche de la distribution de l’annonacine. Thèse de Doctorat, Université Paris-Sud. 2012;11: 40-109.

Olugbuyiro JAO, Omotosho OE, Taiwo OS, Ononiwu FO, Banwo AS, Akintokun OA, Obaseki OS, Ogunleye OM. Antimicrobial activities and phytochemical properties of Annona muricata leaf. Coven J. Phys. Life Sci. 2017;5:40-49.

Tojo OB, Lajide L, Owolabi BJ, Olaleye MT Okoh SO. Phytochemical screening & antibacterial activity of ethyl acetate & methanol extracts of Annona muricata aerial part. Journal of Medicinal Plants Studies. 2019;7(6):1-5.

Silva MA, Alvarenga CD, Bezerra- Silva GCD, Mastrangelo T, Lopes-Mielezaski GN, Giustolin T. Toxic effects of neem seed cake on the larval-pupal (prepupal) stage of Mediterranean fruit fly (Diptera: Tephritidae). Fruits. 2011;66(5): 363-369.

Greuter W, Mcneill J, Barrie FR, Burdet HM , Demoulin V, Filgueiras TS, Nicolson DH, Silva PC, Skog JE, Trehane P, Turland NJ, Hawksworth DL. International code of botanical nomenclature (St. Louis Code). Adopted by the XVth International Botanical Congress St Louis. Koeltz Scientific Books: Königstein; 2003.

Stoll. Protection Naturelle des végétaux en zone Tropicale. CTA. Agrecol. 1994;95-99.

Ondoa F. Effet des extraits aqueux des graines de laurier jaune et des pesticides chimiques sur les maladies des taches foliaires du manioc. Master, Université de Yaoundé I. 2009;39.

Ngoh JP, Ambang Z, Bekolo N, Heu A, Kuate TWN. Effect of extracts of Thevetia peruviana on the development of Phytophthora megakarya causal agent of black disease of Cocoa. J. App. Biosci. 2014;77:6564-6574.

DOI: 10.4314/jab.v77i1.11

Harbone J. Phytochemical methods. A guide to modern techniques of plant analysis Chapman and Hall, London. 1973;150.

Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology. 2005;4(7):685-688.

DOI: 10.5897/AJB2005.000-3127

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: A review. Internationale Pharmaceutica Sciencia. 2011;1(1):98106.

Banu KS, Cathrine L. General techniques involved in phytochemical analysis. International Journal of Advanced Research in Chemical Science. 2015;2(4): 25-32.

Djeugap JF, Fontem DA, Tapondjou AL. Évaluation des milieux de culture pour la croissance de Phytophthora infestans, agent causal du mildiou chez la morelle noire. Biosciences Proceedings. 2009;15: 85-92.

Tsopmbeng NG, Megtche CJP, Lienou JA, Yaouba A, Djeugap FJ, Fontem DA. Evaluation des activités antifongiques des extraits de plantes contre Phytophthora colocasiae, agent causal du mildiou du taro (C. esculentus (L) Schott). J. Appl. Biosci. 2014;81:7221-7232.

DOI: 10.4314/jab.v81i1.2

Ondo AS. Caractérisation de quelques isolats de P. megakarya agent causal de lapourriture brune des cabosses de cacaoyer (Theobroma cacao L). Mémoire de DEA, Université de Yaoundé I. 2005;58.

Nyassé S. Structure d’une population de phytophthora spp. Des cacaoyères camerounaises atteintes de pourriture brune. Mémoire de diplôme de recherche Universitaire ENSAT, Toulouse. 1992;43.

Vaz PDC. IMP Description of Fungi and Bacteria. 1987;92-916.

Hsieh WH, Goh TK. Cercospora and similar fungi from Taiwan. Maw Chang Book Compagny, Taiwan; 1990.

Available:www.bcrc.firdi.org.tw/fungi/fungal

Singh G, Padvay RK, Narayanam CS, Padmhurmeri KP, Rao GP. Chimical and fongistatic investigation out the essential oil Citrus. Pers. Z. dentshe zeeits halft fur pflanzenfrankenen und flanzenschustz. 1993;100:69-74.

Pandey DK, Chandra H, Tripathi NN. Volatile fongitoxicity activity in higher plants special reference to that of Callistemun lanceolatus D.C. Phyto-pathology. 1982;105:175-182.

Kishore N, Mishra AK, Cham SYNN. Fungitoxicity of essential oil against dermatophytes. Mycoses. 1993;36:211-215.

Dohou N, Yamni K, Badoc A, Douira A. Activité antifongique d’extraits de Thymelaea lythroides sur trois champignons pathogènes du riz. Bull. Soc. Pharm. 2004;143:31-38.

Tsopmbeng GR, Lienou JA, Megaptche CJP, Fontem DA. Effect of pH and temperature levels on in vitro growth and sporulation of Phytophthora colocasiae, taro leafl blight pathogen. Int. J. Agro. Agri. Resch. 2014;4(4):202-206.

Muhammad Z, Sadia H, Komal R, Nasir R, Muhammad R, Zia-Ul-Haq M, Vincenzo DF. Antioxidant potential and oil composition of Callistemon viminalis leaves. Scientific World Journal. 2013;10: 11-55.

DOI: 10.1155/2013/489071

Bruneton J. Phytochimie, Plantes médicinales. 3e édition Tec. et Doc., Lavoisier Paris. 1999;11-20.

Smallfield B. Introduction to growing herbs for essential oils, medicinal and culinary purposes. Crop & Food Research. 2001; 45:1-4.

Valnet J. Aromatherapie: Traitement des maladies par les essences des plantes. 9e Ed. Maloine. 1980;510.

Omolara JO, Matthew OO, Abiola MA. Comparative phytochemistry and antioxidant activities of water and ethanol extract of Annona muricata leaf seed and fruit. Journal of Advances in Biological Research. 2016;10(4):230-235.

Naik AV, Sellappan K. Physiochimical and phytochemical Analysis of different plant parts of Annona muricata L. (Annonaceae). Pharm Methods. 2019;10(2):70-78.

DOI: 10.5530/phm.2019.2.13

Pamo TE, Tapondjou L, Temdonkeng F, Nzogang JF, Djoukeng J, Ngandeu F, Kana JR. Effet des huiles essentielles des feuilles et des extrémités fleuries des Cupressus lussitanica sur la Tique (Rhipicephalus Lunulatus) à l’ouest Cameroun. Revue de l’Académie des Sciences du Cameroun. 2003;3(3):169-175.

Kone NAN, Ndongo B, Mountapmbeme MM, Manga EFR, Heu A, Mvondo ND, Mboussi SB, Ambang Z. Anti-fungal activities of Jatropha curcas seeds extracts against Cercospora malayensis causative agent of Sigatoka of okra leaves. Inter. J. Sc. Resc. Methd. 2018;9(1):95-109.

Bautista BH, Lopez M, Bosquez ME, Wilson CL. Effect of extracts and plant extracts on growth of Colletotricum gloeosporioides, anthracnose and quality of papaya fruit. Crop Protection. 2003; 1087-1092.

Gata-Gonçalves L, Nogueira JMF, Matos O, De Sousa BR. Photoactive extract from Thevetia peruviana with antifungal properties against Cladosporium cucumerinum. J. Photochem Photobiol B. 2003;70(1):51-54.

DOI: 10.1016/s1011-1344(03)00024-1

  • 136 times
    PDF Download: 21 times

Download Statistics

Downloads

Download data is not yet available.
Make a Submission

Information

Current Issue