Antibiotics Profile and Public Health Implication of Pathogenic Enteric Bacteria Associated With Poultry Stool
International Journal of Pathogen Research, Volume 12, Issue 2,
Page 9-15
DOI:
10.9734/ijpr/2023/v12i2221
Abstract
Poultry waste are not properly disposed and most rural farmers make use of it as manure therefore poultry wastes may serve as source of enteric organism capable of infecting humans. The antibiotic resistance from these bacteria can be transferred to natural microbial community as a result of involvement of antibiotics in poultry farming. This research was carried out to assess the multidrug resistant pattern of enteric bacteria in poultry stool dropping. samples of poultry droppings were obtained from a free-range commercial poultry farm in Otuoke, Bayelsa State, Nigeria. Samples were cultured on selective and differential media. 16 isolates of enteric bacteria were obtained from poultry stool droppings. The isolates identified were of four genera. Salmonella species which is predominant 6(37%) followed by Escerichia coli 5(31%) Proteus species 3(19%) and Kiebsiella species 2(13%). Antibiotic susceptibility testing was carried out using the disk diffusion technique. Gram negative antibiotics including crioxacin cefprozil, nitrofuration, ceftazidime co-trimoxazole, genetamicin, cefuroximo. The resistant pattern in among the gram negative pathogens revealed that more than 98% were resistant to nitrofuratioin cefprozil, gentamicin and augumentin while 12.89% resistant to certazidime, 90.10% resistant to augmentin, 15.32% cefuroxime, 12.30% co-trimoxazole, 38.17% resistant to gentamicin, 15.20% resistant to cefprozil and 50.20% resistant to ofloxacin, Multidrug resistance species were isolated from poultry stool dropping and some of the bacterial isolates are potentially pathogenic to humans and animals and therefore poses a serious threat to public health.
- Multidrug resistance
- poultry
- enteric bacteria
- stool dropping
How to Cite
References
Bolan NS, Szogi AA, Chuasavatti T, Seshadri B, Rothrock MJ, Jr, Panneerselvan P. Uses and management of poultry litter. World’s Poult. Sci. J. 2010;66:673-698.
Wilkinson KG, Tee E, Tomkins RB, Hepworth G, Premier R. Effect of heating and aging of poultry litter on the persistence of enteric bacteria. Poult. Sci. 2011;90:10-18.
Stern NJ, Robaeh MC. Enumeration of Campylobacter Spp in broiler feaces and in corresponding processes carcasses. J. Food Protect. 2003;66:1557-1563.
Ngodigha EM, Owen OJ. Evaluation of the bacteriological characteristics of poultry litters as feedstuff for cattle. Sci. Res. Essays. 2009;4:188-190.
Catry B, Laevens H, Duriese LA, Opremer G, Dekruif A. Antimicrobial resistance in Livestock. S. Vet Pharmacol Therapy. 2003;26:81- 93.
Madigan MT, Martinko SM, Buckley FH, Stall DA. Brook Biology of Microorganisms 14th ed. Illinois: Pearson International. 2014;1006.
Van Den Bogaerd AE, Stobberungh EE. Epidemology of resistance to antibiotic link between animals and human. Int J. Antimicrob Agents. 2000;14:327- 335.
Amara A, Ziaru Z, Bouzoubaa K. Antibiotic resistance of Escherichia coli Strains isolated in Morocco from chicken with Colibacillosis. Vet Microbial. 1995;43:325-330.
Gupta K, Hooton TM, Stamm WE. Increasing antimicrobial resistance and the management of uncomplicated community acquired urinary tract infection. Ann Int. Med. 2001;135: 41-50.
Mitchell MA. and Shane SM. Preliminary findings of Salmonella spp. in captive green iguanas (Iguana iguana) and their environment. Prev. Vet. Med. 2000;45: 297-304.
Al-Abadi IKM, AL- Mayah AAS. Isolation and identification of Salmonella spp. from chicken and chicken environment in Basrah province. African J. Biol. Sci. 2011;7 (1):33-43.
Golden CE, Rothrock MJ, Mishra A. Mapping foodborne pathogen contamination throughout the conventional and alternative poultry supply chains. Poult. Sci. 2021;100:101157.
Pujiastuti ES, Tarigan JR, Sianturi E, Ginting BB. The effect of chicken manure and beneficial microorganisms of EM-4 on growth and yield of kale (Brassica oleraceae acephala) grown on Andisol. IOP Conf. Ser. Earth Environ. Sci. 2018;205:012020.
Merchant LE, Rempel H, Forge T, Kannangara T, Bittman S, Delaquis P, Topp E, Ziebell KA, Diarra MS. Character-ization of antibiotic-resistant and potentially pathogenic Escherichia coli from soil fertilized with litter of broiler chickens fedantimicrobial-supplemented diets. Can. J. Microbiol. 2012;58:1084–1098.
Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing: twenty-fourth informational supplement. CLSI document M100-524 Wayne, PA:50-84; 2014.
Bamidele O, Yakubu A, Joseph EB, Amole TA. Antibiotic resistance of bacterial isolates from smallholder poultry droppings in the guinea savanna zone of Nigeria. Antibiotics. 2022;11:973.
Mansaray AHD, Yankson DPY, Johnson RAB, Moses FL, Kanu JS, Kamara IF, Zachariah R, Kumar AMV, Selvaraj K. bacterial isolates and antibiotic resistance of escherichia coli isolated from fresh poultry excreta used for vegetable farming in freetown, Sierra Leone. Int. J. Environ. Res. Public Health. 2022;19: 5405.
Abdu HU. Isolation and characterization of multidrug-resistant Escherichia coli from poultry litter samples from selected farmsin Kano metropolis, Nigeria. Niger. J. Microbiol. 2021;35:5623–5629.
Amadi VA, Watson N, Onyegbule OA, Matthew-Belmar V, Avendano E, Tiwari K, Sharma R, Hariharan H. Antimicrobial resistance profiles of Escherichia coli recovered from feces of healthy free-range chickens in Grenada, West Indies. Int. J. Curr. Microbiol. Appl. Sci. 2015;4:168– 175.
Vounba P, Yaghouba K, Ndiaye C, Arsenault J, Fairbrother JM, Bada AR. Molecular characterization of Escherichia coli isolated from chickens with colibacillosis in Senegal. Foodborne Pathog. Dis. 2018;15:517–525.
Moffo F, Mouiche MMM, Djomgang HK, Tombe P, Wade A, Kochivi FL, Dongmo JB, Mbah CK, Mapiefou NP, Ngogang MP, et al. Poultry litter contamination by Escherichia coli resistant to critically important antimicrobials for human and animal use and risk for public health in Cameroon. Antibiotics. 2021;10: 402.
Eyasu A, Moges F, Alemu A. Bacterial isolates from poultry litters and their antimicrobial susceptibility patterns in Gondar, Northwest Ethiopia. Int. J. Microbiol. Res. Rev. 2017;6:197–204.
Joseph FN. Prevalence and antibiotic resistance of Escherichia coli O157: H7 serotype from chicken droppings produced by free—ranged and poultry birds in Cross River, Nigeria. Am. J. Biomed. Life Sci. 2018;6: 51.
Adelowo OO, Fagade OE, Agersø Y. Antibiotic resistance and resistance genes in Escherichia coli from poultry farms, southwest Nigeria. J. Infect. Dev. Ctries. 2014;8:1103–1112.
Kilonzo-Nthenge A, Nahashon SN, Chen F, Adefope N. Prevalence and antimicrobial resistance of pathogenic bacteria in chicken and guinea fowl. Poultry Science. 2008;87(9):1841- 1848.
-
Abstract View: 29 times
PDF Download: 26 times
