The Use of Immersion Oil in Parasitology Light Microscopic Examination

Forman Erwin Siagian *

Department of Parasitology, Faculty of Medicine, Universitas Kristen Indonesia, Jakarta, Indonesia.

*Author to whom correspondence should be addressed.


Aims: to briefly review the history of application of immersion oil and specifically in some Parasitology Microscopic examination.

Discussion: One way of increasing the optical resolving power of the microscope is to use immersion liquids between the front lens of the objective and the cover slip. The application of immersion oil in microscopic examination began in the mid-17th century. The principle of immersion oil in microscopic examination is to improve the resolution and clarity of the image by reducing the refractive index mismatch between the specimen and the objective lens. In the context of micro-sized parasitic agent, adding this oil will greatly enhanced visualization and in turn will have a direct impact on improving the characteristic morphological image/appearance, thereby enabling observers to optimize microscopic assessment for parasitological examination. Protozoan parasites such as Plasmodium spp, Entamoeba histolytica, Giardia lamblia, Microsporidia spp., Trichomonas vaginalis, Toxoplasma spp, Trypanosoma spp, Babesia spp, Leishmania spp, Cyclospora cayetanensis, and Blastocystis hominis are some examples of parasitic organisms that should be viewed at great magnification to see detailed morphology; and for that it means you need to add immersion oil.

Conclusion: The addition of immersion oil in Paarasitology microscopic examination is surely improve optical resolution and clarity of the image and positively support Parasitologist to make correct diagnosis.

Keywords: Resolution, magnification, lens, numerical aperture object, micro-parasite, protozoan, morphology

How to Cite

Siagian , F. E. (2024). The Use of Immersion Oil in Parasitology Light Microscopic Examination . International Journal of Pathogen Research, 13(2), 1–8.


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Schädler K. An Introduction to the Light Microscope, Light Microscopy Techniques and Applications [Internet]. Tedhnology Networks; 2021 Sept, 2. Available:

Bicchieri M, Biocca P, Colaizzi P, Pinzari F. Microscopic observations of paper and parchment: the archaeology of small objects. Herit Sci. 2019;7:47. Available:

Rouzie D, Lindensmith C, Nadeau J. Microscopic Object Classification through Passive Motion Observations with Holographic Microscopy. Life. 2021;11(8):793. Available:

Antony PM, Trefois C, Stojanovic A, Baumuratov AS, Kozak K. Light microscopy applications in systems biology: opportunities and challenges. Cell Commun Signal. 2013 Apr 11;11(1):24. Available:

Heintzmann R, Ficz G. Breaking the resolution limit in light microscopy, Briefings in Functional Genomics. 2006;5(4):289–301. Available:

Alberts B, Johnson A, Lewis J. Molecular Biology of the Cell. 4th edition. New York: Garland Science; 2002. Looking at the Structure of Cells in the Microscope. Available:

Sacher R. Microscope Immersion Oil. Microscopy Today. 2000;8:33-5. Available:

Davidson M. Pioneers in Optics: Zacharias Janssen and Johannes Kepler. Microscopy Today; 2009. Available:

Hooke R. Lectures and Collections. Downloaded from

Greenberg SJ. Hooke’s books: influences around Robert Hooke’s micrographia. Jul, 23 2020. Downloaded from

Aamir S, Haque A, Zahid M. Utility of Oil Immersion Lens in Evaluation of Malignancy. International Journal of Pathology. 2005;3(1):1-5.

Britannica, T. Editors of Encyclopaedia. "Giovanni Battista Amici." Encyclopedia Britannica; 2023. Available:

Davidson MW. Pioneers in Optics: Giovanni Battista Amici and Girolamo Cardano. Microscopy Today. 2010;18(3):50–2. Available:

American Optical Company. Scientific Instrument Division. Three American microscope builders; 1945. Available:

Krauss WC. The Debt of American Microscopy to Spencer and Tolles. Transactions of the American Microscopical Society. 1902;23:19–30. Available:

Kirkpatrick L, Wheeler G. Physics: A World View (2nd ed.). Philadelphia: Harcourt Brace College Publishers. 1992;410. ISBN 0-03-000602-3.

Su R, Thomas M, Liu M, Bellouard Y, Pruss C, Coupland J, et al. Lens aberration compensation in interference microscopy. Optics and Lasers in Engineering. 2020;128:Article 106015. Available:

Ford BJ. Turning back the centuries of microscopy. Wiley Analytical Science newsletter. 2022, Dec 14.

Downloaded from

Wilson M. How History Shaped Modern Optical Microscopes, Part Two: Corrected Lenses and Objectives. BITESIZE BIO. 2021, April 13. Downloaded from,and%20(5)%20field%20curvature.

Allen T. Microscopy and the discovery of a new world', Microscopy: A Very Short Introduction, Very Short Introductions (Oxford, 2015; online edn, Oxford Academic, 28 May 2015). Available:

Rottenfusser R, Wilson EE, Davidson MW. Historical Perspective of Optical Microscopy. Downloaded from

Davidson MW, Abramowitz M. Optical Microscopy. Downloaded from

Birk U, Hase Jv, Cremer C. Super-resolution microscopy with very large working distance by means of distributed aperture illumination. Sci Rep. 2017;7:3685. Available:

Anonymous. Increase Resolution with Immersion Oil. Microscope World /Blog / Microscope Immersion Oil [online]. Downloaded from,air%20as%20the%20imaging%20medium.

Cargille JJ. Immersion oil and the microscope. New York Microscopical Society Yearbook, 1964. Second Edition ©1985 downloaded from

Rottenfusser R, Wilson EE, Davidson MW. Numerical aperture and resolution. Downloaded from

Ibrahim HI, Kafi SK. Using Water with Oil Immersion Lens to Detect Malaria Parasite in Blood Film and Making a Comparison between Oil and Water Method. American Journal of Microbiological Research. 2014;2(4):122-4. Available:

McDougall AC. The use of xylene (xylol) in medical laboratories. Lepr Rev. 1989 Mar;60(1):67.

Petrak LJ, Waters JC. A practical guide to microscope care and maintenance. Methods Cell Biol. 2014;123:55-76. Available:

Waters J. Preventing Objective Lens Damage: Immersion Oil Problems [Internet]. Microcourses. 2018, Nov 20. Video: duration 7:50. Available:

Centonze Frohlich V. Proper care and cleaning of the microscope. J Vis Exp. 2008 Aug 11;(17):842. Available:

Milgroom MG. Protozoa. In: Biology of Infectious Disease. Springer, Cham; 2023. Available:

Mehlhorn H. Balantidium coli. In: Mehlhorn, H. (eds) Encyclopedia of Parasitology. Springer, Berlin, Heidelberg; 2016. Available:

Zhang C, Jiang H, Jiang H, Xi H, Chen B, Liu Y, Juhas M, Li J, Zhang Y. Deep learning for microscopic examination of protozoan parasites. Comput Struct Biotechnol J. 2022 Feb 11;20:1036-1043. Available:

Mulenga GM, Namangala B, Chilongo K, Mubamba C, Hayashida K, Henning L, Gummow B. Challenges in the Diagnostic Performance of Parasitological and Molecular Tests in the Surveillance of African Trypanosomiasis in Eastern Zambia. Trop Med Infect Dis. 2021 Apr 30;6(2):68. Available:

Institute of Medicine (US) Committee for the Study on Malaria Prevention and Control; Oaks SC Jr., Mitchell VS, Pearson GW, et al., editors. Malaria: Obstacles and Opportunities. Washington (DC): National Academies Press (US); 1991. 6, Parasite Biology. Available:

Das S, Rajkumari N, Gunalan A, Rajavelu D, Olickal JJ. A Comparative Analysis of Microscopy, Coproantigen Serology, and Nested Multiplex PCR in the Laboratory Diagnosis of Entamoeba histolytica Infection. J Lab Physicians. 2021 Jul 29;14(2):125-131. Available:

Rajurkar MN, Lall N, Basak S, Mallick SK. A simple method for demonstrating the giardia lamblia trophozoite. J Clin Diagn Res. 2012 Nov;6(9):1492-4. Available:

Fadhilah A, Gabbar A, Bokhari AA. Microsporidium. [Updated 2023 Aug 28]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2024 Jan-. Available:

Gerace E, Lo Presti VDM, Biondo C. Cryptosporidium Infection: Epidemiology, Pathogenesis, and Differential Diagnosis. Eur J Microbiol Immunol (Bp). 2019 Oct 22;9(4):119-123. Available:

Cheon SH, Kim SR, Song HO, Ahn MH, Ryu JS. The dimension of Trichomonas vaginalis as measured by scanning electron microscopy. Korean J Parasitol. 2013 Apr;51(2):243-6. Available:

Mayoral J, Di Cristina M, Carruthers VB, Weiss LM. Toxoplasma gondii: Bradyzoite Differentiation In Vitro and In Vivo. Methods Mol Biol. 2020;2071:269-282. Available:

Kalichava A, Ochsenreiter T. Ultrastructure expansion microscopy in Trypanosoma brucei. Open Biol. 2021 Oct;11(10): 210132. Available:

Petersen B, Ahmed JS. Babesia . In: Mehlhorn H. (eds) Encyclopedia of Parasitology. Springer, Berlin, Heidelberg. 2016. Available:

Gow I, Smith N, Stark D, Ellis J. Laboratory diagnostics for human Leishmania infections: a polymerase chain reaction-focussed review of detection and identification methods. Parasites & Vectors. 2022;15. Available:

Mathison BA, Pritt BS. Cyclosporiasis-Updates on Clinical Presentation, Pathology, Clinical Diagnosis, and Treatment. Microorganisms. 2021 Sep 2;9(9):1863. Available:

Beyhan YE, Yilmaz H, Cengiz ZT, Ekici A. Clinical significance and prevalence of Blastocystis hominis in Van, Turkey. Saudi Med J. 2015 Sep;36(9):1118-21. Available: