Growth and Survival of Enterotoxigenic Escherichia coli (ETEC) Isolated from Tuna Loins Produced in Côte D’ivoire
International Journal of Pathogen Research,
Aims: The aim of this study was to study the growth of pathogenic strains of Escherichia coli (ETEC) in Tuna loins.
Study Design: Bacteriological study.
Place and Duration of Study: Laboratory of Microbiology of the Central Laboratory of Food hygiene and Agro-business (LCHAI), Abidjan, Côte d’Ivoire between September 2014 and December 2014.
Methodology: Three strains of E. coli (enterotoxigenic strain of E. coli (ETEC), possessing both "elt" and "est" virulence genes resistant to amoxicillin from Tuna loins; E. coli reference strain (ATCC 25992); strain of E. coli (KO 13) from water with the virulence gene "elt") were inoculated in brain heart infusion broth (BHI) and in tuna loins for 120 hours. pH and bacterial loads of E. coli were measured to 0; 3; 6; 12; 24; 48; 72; 96 and 120 hours respectively.
Results: The results showed that the three strains of E. coli used in this work survived in liquid medium (BHI) and in Tuna loins even after 5 days (120 hours). The growth curves of these three strains evolved in the same way in liquid medium and in Tuna loins. However, the growth rate of strains of E. coli inoculated in liquid media (BHI) was higher than that of strains inoculated in Tuna loins.
Conclusion: Pathogenic strains of E. coli isolated from Tuna loins are a hazard to be considered in the microbiological risk assessment of the consumption of these Tuna products.
- Enterotoxigenic Escherichia coli (ETEC)
- tuna loins
- Brain Heart Infusion broth (BHI)
How to Cite
Chahed A, China B, Daube G. Les Escherichia coli producteurs de Shigatoxins dans les toxi-infections d’origine alimentaire, Annales de Médecine Vétérinaire. 2007;151:215-246.
Bradley K, Williams J, Burnsed L, Lytle M, McDermott M, Mody R, Bhattarai A, Mallonee S, Piercefield E, McDonald-Hamm C, Smithee L. Epidemiology of a large restaurant-associated outbreak of Shiga toxin producing Escherichia coli O111: NM. Epidemiology and Infection. 2012;140:1644–1654.
Dallman T, Smith G, O'Brien B, Chattaway M, Finlay D, Grant K, Jenkins C. Characterization of a verocytotoxin-producing enteroaggregative Escherichia coli serogroup O111: H21 associated with a household outbreak in Northern Ireland. Journal of Clinical Microbiology. 2012;50 (12):4116–4119.
Loukiadis E, Kerouredan M, Beutin L, Oswald E. Brugere. Characterization of Shiga toxin gene (stx)-positive and intimin gene (eae)-positive Escherichia coli isolates from wastewater of slaughterhouses in France. Applied Environmental Microbiology. 2006;72: 3245-3251.
Qadri F, Svennerholm A, Faruque A, Sack R. Enterotoxigenic Escherichia coli in developing countries: Epidemiology, microbiology, clinical features, treatment, and prevention. Clinical Microbiology Reviews. 2005b;18:465-484.
Teophilo G, Vieira R, Rodrigues D, Menezes F. Escherichia coli isolated from seafood: toxicity and plasmid profiles. International Microbiology, 2002;5:11–14.
Levine M. Escherichia coli that cause diarrhea: Enterotoxigenic, enteropathogenic, enteroinvasive, enterohemorrhagic and enteroadherent. Journal of Infectious Diseases. 1987;155: 377-389.
Nataro J, Kaper J. Diarrheagenic Escherichia coli. Clinical Microbiology Reviews. 1998;11:142-201.
DPH (Direction of Halieutic Production). Annuaire des statistiques des pêches et de l’aquaculture. Service des études, des statistiques et de la documentation. Document Technique. 2014;36.
Sika AE, Kambire O, Zamblé Bi IAB, Aké-Assi Y, Koffi-Nevry R. Virulence genes and antibiotic resistance profile of Escherichia coli strains isolated from tuna loins and flakes produced in Côte d’Ivoire. International Journal of Current Microbiology and Applied Sciences. 2018; 7(9):3329-3338.
Cho G, Lee M, Choi C. Survival of Escherichia coli O157:H7 and Listeria monocytogenes during kimchi fermentation supplemented with raw pork meat. Food Control. 2011;22:1253-1260.
Zwietering MH, Jongenburger I, Rombouts FM, van’t Rict K. Modelling of the bacterial growth curve. Applied of Environmental Microbiology. 1990;56:1875–1881.
Ongeng D, Geeraerd AH, Springael D, Ryckeboer J, Muyanja C, Mauriello G. Fate of Escherichia coli O157:H7 and Salmonella enterica in the manure-amended soil-plant ecosystem of fresh vegetable crops: a review. Critical Reviews of Microbiology. 2015;41:273–294.
NandaKafle G, Christie AA, Vilain S, Brözel VS. Growth and Extended Survival of Escherichia coli O157:H7 in Soil Organic Matter. Frontiers in Microbiology, 2018;9: 762.
Chauret C. Survival and control of Escherichia coli O157:H7 in foods, beverages, soil and water. Virulence. 2011; 2(6):593-601.
Kudva IT, Blanch K, Hovde CJ. Analysis of Escherichia coli O157:H7 survival in ovine or bovine manure and manure slurry. Applied of Environmental Microbiology. 1998;64:3166–3174.
Jiang X, Morgan J, Doyle MP. Fate of Escherichia coli O157:H7 in manure-amended soil. Applied Environmental Microbiology. 2002;68:2605–2609.
Vital M., Hammes F., Egli T. Escherichia coli O157 can grow in natural freshwater at low carbon concentrations. Environmental of Microbiology. 2008;10: 2387–2396.
Elsas JD, Semenov AV, Costa R, Trevors JT. Survival of Escherichia coli in the environment: fundamental and public health aspects. The International Society for Microbial Ecology Journal. 2011;5:173–183
Chang JM, Fang TJ. Survival of Escherichia coli O157:H7 and Salmonella enterica serovars Typhimurium in iceberg lettuce and the antimicrobial effect of rice vinegar against Escherichia coli O157:H7. Food Microbiology. 2007;24:745-751
Aruscavage D, Miller S, Lewis Ivey ML, Lee K, LeJeune JT. Survival and dissemination of Escherichia coli O157:H7 on physically and biologically damaged lettuce plants. Journal of Food Protection. 2008;71:2384-2388
Alegre I, Abadias M, Anguera M, Usall J, Vinas I. Fate of Escherichia coli O157:H7, Salmonella and Listeria innocua on minimally-processed peaches under different storage conditions. Food Microbiology. 2010;27:8628.
Strawn LK, Danyluk MD. Fate of Escherichia coli O157:H7 and Salmonella spp on fresh and frozen cut mangoes and papayas. International Journal of Food Microbiology. 2010;138:78-84.
Raybaudi-Massilia RM, Mosqueda-Melgar J, SobrinoLopez A, Soliva-Fortuny R, Martin-Belloso O. Use of malic acid and other quality stabilizing compounds to assure the safety of fresh-cut “Fuji” apples by inactivation of Listeria monocytogenes, Salmonella enteritidis and Escherichia coli O157:H7. Journal of Food Safety. 2009;29: 236-252.
Brooks JC, Alvarado M, Stephens TP, Kellermeier JD, Tittor AW, Miller MF. Spoilage and safety characteristics of ground beef packaged in traditional and modified atmosphere packages. Journal of Food Protection. 2008;71:293-301.
Vernozy-Rozand C, Mazuy-Cruchaudet C, Bavai C, Montet M.P., Bonin V., Dernburg A., Richard Y. Growth and survival of Escherichia coli O157:H7 during the manufacture and ripening of raw goat milk lactic cheeses. International Journal of Food Microbiology. 2005;105:83–88.
Dixon L, Donnison A, Harfoot C, Mcdonald I. Survival of Escherichia coli in toroi: A traditional Māori food. New Zealand Journal of Marine and Freshwater Research. 2007; 41:369-375.
Tassou C, Samaras F, Arkoudelos J, Mallidis S. Survival of acid-adapted or non-adapted Salmonella Enteritidis, Listeria monocytogenes and Escherichia coli O157:H7, in traditional Greek salads. International Journal of Food Science and Technology. 2009;44:279–287
Berry E, Cutter C. Effects of acid adaptation of Escherichia coli O157:H7 on efficacy of acetic acid spray washes to decontaminate beef carcass tissue. Applied Environmental Microbiology. 2000;66:1493-1498.
Gullian-Klanian M, Sánchez-Solis MJ. Growth kinetics of Escherichia coli O157: H7 on the epicarp of fresh vegetables and fruits. Brazilian Journal of Microbiology. 2018;49(1):104-111.
Vimont A, Vernozy-Rozand C, Delignette-Muller M. Isolation of E. coli O157: H7 and non O157:H7 in different matrices; Review of the most commonly used enrichment protocols. Letters in Applied Microbiology. 2006;42:102- 108.
Rodriguez R, Inglis V, Millar S. Survival of Escherichia coli in the intestine of fish. Aquaculture Research. 1997;28:257- 264.
Abstract View: 820 times
PDF Download: 518 times