Activities of Macrolides in Combination with Vitamin D against Bacillus cereus Isolated from Soil of a Lanfill Site in Nsukka South East Nigeria

Main Article Content

Ekwebelem C. Osmond
Nnorom-Dike V. Obinna

Abstract

Background: The fast moving world of antibiotics resistance is now a big concern, since our antibiotics are in danger of extinction. This is a concern to us because; an empty ‘antibiotic pipeline’ leads to devastating future.

Aim: The aim of this study was to isolate Bacillus cereus from soil contaminated with various toxic and domestic wastes and investigate its sensitivity to macrolide antibiotics (erythromycin ERY and azithromycin AZM) in combination with vitamin D supplement.

Methodology: Five soil samples were collected from a landfill site and serially diluted up to 10ˉ6. B. cereus was isolated from the soil sample using Mannitol egg Yolk Polymyxin agar medium (MYP), a selective nutrient medium for B. cereus isolation. The sensitivity of B. cereus to erythromycin and azithromycin was screened in Mueller-Hinton agar (MHA) containing varying concentrations (5, 10, 20 mg/ml) of vitamin D. Agar without vitamin D (0.00mg/ml) served as the control. The activities of ERY and AZM in combination with vitamin D were determined by measuring the zones of inhibition.

Results: In vitro antibiotic susceptibility tests on our isolate showed maximum sensitivity to ERY and maximum resistance to AZM with 0.00 mg/ml (no combination) of vitamin D. Contrarily, ERY was found to be least active, with the zones of inhibitions decreasing from 28mm to 16mm, with increasing concentration of vitamin D. On the other hand, AZM achieved maximum sensitivity from 12mm to 25mm, with increasing concentration of vitamin D.

Conclusion: The activities appeared to be important but dose-dependent. So, the systematic use of Vitamin D in combination with macrolide antibiotics may be efficient in treating food and soil infections caused B. cereus. However, in vivo evaluation of these activities is needed.

Keywords:
Erythromycin, azithromycin, Vitamin D, Bacillus cereus, landfill, soil

Article Details

How to Cite
Osmond, E. C., & Obinna, N.-D. V. (2020). Activities of Macrolides in Combination with Vitamin D against Bacillus cereus Isolated from Soil of a Lanfill Site in Nsukka South East Nigeria. International Journal of Pathogen Research, 5(1), 10-15. https://doi.org/10.9734/ijpr/2020/v5i130123
Section
Original Research Article

References

Kotiranta A, Lounatmaa K, Haapasalo M. Epidemiology and pathogenesis of Bacillus cereus infections. Microbes Infect. 2000; 2:189–198.

Pushpendra S, Rajesh S, Ashish KS, Ravindra S. Isolation of Bacillus spp. from Soil for Antimicrobial Production and Antibiotic Resistance. Adv Biotech Micro. 2018;8(4):555741.

DOI: 10.19080/AIBM.2018.08.555741

Marrollo R. Bacillus cereus food-borne disease, in The Diverse Faces of Bacillus cereus, ed. V. Savini (London: Academic Press). 2016;61–72.

Hong HA, Duc Le H, Cutting SM. The use of bacterial spore formers as probiotics. FEMS Microbiol Rev. 2005;29:813–835.

Lund T, De Buyser ML, Granum PE. A new cytotoxin from Bacillus cereus that may cause necrotic enteritis. MolMicrobiol. 2000;38:254–261.

Dierick K, Van Coillie E, Swiecicka I, Meyfroidt G, Devlieger H, Meulemans A, et al. Fatal family outbreak of Bacillus cereus associated food poisoning. J ClinMicrobiol. 2005;43:4277–4279.

Ehling-Schulz M, Fricker M, Scherer S. Bacillus cereus, the causative agent of an emetic type of food-borne illness. MolNutr Food Res. 2004;48:479–487.

Beecher DJ, MacMillan JD. Characterization of the components of hemolysin BL from Bacillus cereus. Infect Immun. 1991;59:1778–1784.

Lund T; Granum PE. Characterisation of a nonhaemolytic enterotoxin complex from Bacillus cereus isolated after a foodborne outbreak. FEMS Microbiol Lett. 1996;141: 151–156.

Arnesen LPS, Fagerlund A, Granum PE. From soil to gut: Bacillus cereus and its food poisoning toxins. FEMS Microbiol Rev. 2008;32:579–606.

Bikle D. Vitamin D and the immune system Curr Opin Nephrol Hypertens. 2008;17: 348–352.

Youssef DA, Miller CT, El-Abbassi A, Cutchins DC, Cutchins C, Grant WB et al. Antimicrobial implications of vitamin D. Dermatoendocrinol. 2011;3(4):220-229.

Majed M, Mhaidat NM, Alzoubi KH, Al-Azzam SI, Shaweesh AI. Ciprofloxacin-Induced Antibacterial Activity is Reversed by Vitamin E and Vitamin C. Curr Microbiol, 2012;64(5):457-462.

Anderson-Cook CM, Alley MM, Roygard JK, Khosla R, Noble RB, Doolittle JA. Differentiating soil types using electromagnetic conductivity and crop yield maps. Soil SciSoc of Am J. 2002;66:1562-1570.

Kaur M, Samyal M, Ahmed Z, Kaur A, Isolation characterization and identification of bacterial strain producing amylase. J Microbiol Biotech Res. 2012;2(4):573-579.

Boyan B, Hooper J, Parisot J. Principles of assessing bacterial susceptibility to antibiotics using the agar diffusion method. Journal antimicrobchemother. 2008;61(6): 1295-1301.

Bauer AW, Perry DM, Kirby WM. Single-Disk antibiotic-sensitivity testing of Staphylococci an analysis of technique and results. Arch Intern Med. 1959;104(2):208-216.

Clinical and Laboratory Standards Institute (CLSI). Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria; approved guideline, 2nd edn. M45A2E. Wayne: CLSI; 2010.

Chandan P, Mishra RP, Asif A, Gangwar VS, Shweta C. Isolation and characterization of multi drug resistant super pathogens from soil samples collected from hospitals. Res J Recent Sci. 2012;2:124-129.

Yu S, Yu P, Wang J, Li C, Guo H, Liu C, et al. A Study on Prevalence and Characterization of Bacillus cereus in Ready-to-Eat Foods in China. Front. Microbiol. 2020;10:3043.

DOI: 10.3389/fmicb.2019.03043

Banerjee M, Nair GB, Ramamurthy T. Phenotypic& genetic characterization of Bacillus cereus isolated from the acute diarrhoeal patients. Indian J Med Res. 2011;133:8895.

Bikle DD. Vitamin D and the immune system: role in protection against bacterial infection. Curr Opin Nephrol Hypertens 2008;17:348–352.

Biswas S, Thomas N, Mandal A, Chandra D, Mukherjee S. Analysis of antimicrobial activity of vitamin C alone and in combination with antibiotics on Gram positive rod isolated from soil of a dumping site of Kolkata. Int J Pharm Biol Sci. 2013; 3:101-110.

Lohsen S. Stephens DS. Current macrolide antibiotics and their mechanisms of action. Antibiotic Drug Resistance. 2019;97-117.

Verghese RJ, Mathew SK, David A. Antimicrobial activity of Vitamin C demonstrated on uropathogenic Escheriachia coli and Klebsiella pneumoniae. J Curr Res Sci Med. 2017; 3(2):88-93.

Isela S, Sergio N, Jose M, Rene H, Claudio C. Ascorbic acid on oral microbial growth and biofilm formation. Pharm Inno. 2013;2:104-109.

Zhang HM, Wakisaka N, Maeda O, Yamamoto T. Vitamin C inhibits the growth of a bacterial risk factor gastric carcinoma: Helicobacter pylori. Cancer.1997;80:1897-903.

Vilcheze C, Hartman T, Weinrick B, Jacobs WR. Mycobacterium tuberculosis is extraordinarily sensitive to killing by a vitamin C-induced Fenton reaction. Nat Comm. 2013;1: 14-26.