Antifungal Activity on the Strain of Lasiodiplodia theobromae and Phytochemical Study of Ageratum conyzoides and Newbouldia laevis from the Kisangani Region / DR Congo

Main Article Content

J. T. K. Kwembe
J. P. Mbula
O. Onautshu
P. T. Mpiana
G. Haesaert

Abstract

Aims: To extract, identify and evaluate in vitro the antifungal activity of the phytochemical groups of Ageratum conyzoides and Newbouldia laevis on the strain of Lasiodiplodia theobromae.

Study Design: Exploitation of medicinal plants to combat the growth of L. theobromae, responsible for the decline of cocoa cultivation.

Location and Duration of Studies: Faculty of Sciences, University of Kisangani, between April 2017 and February 2018.

Methodology: The crude extracts of the dry leaves of A. conyzoides and N. laevis were tested (at 100 mg/mL). Potato dextrose agar was used as the culture medium. After chemical screening, abundant phytochemical groups were isolated and tested.

Results: The aqueous, 95% ethanolic and ethereal crude extracts of A. conyzoides are more antifungal (respective percentages of inhibition PI: 80.74; 84.10 and 85.64%) than those of N. laevis (63.28; 72.64 and 75.23%). The minimum inhibitory concentration (MIC) of the aqueous crude extract of A. conyzoides is lower (25 mg/mL) than that of the ethanolic extract (50 mg/mL). Tannins are very abundant in A. conyzoides and in N. laevis. Saponins, sterols and terpenes are abundant in both plants. The extraction yields of tannins and saponins are respectively 20.67 and 2.43% in A. conyzoides and 10.47 and 2.38% in N. laevis. A. conyzoides contains the gallic tannins while N. laevis, the condensates and catechics. The saponins and tannins of A. conyzoides are more antifungal (respective PI: 84.40 and 54.44%) than those of N. laevis (PI: 75.56 and 32.96%).

Discussion: The saponins of A. conyzoides and N. laevis are more active on the strain of L. theobromae than the tannins. Saponins are surfactants that can destabilize membrane structure of microorganisms including fungi.

Conclusion: The saponins of the two plants have shown a very interesting antifungal power on the strain of L. theobromae. The identification of their active molecules is ongoing.

Keywords:
Antifungal, Lasiodiplodia theobromae, Ageratum conyzoides, Newbouldia laevis, phytochemical.

Article Details

How to Cite
Kwembe, J. T. K., Mbula, J. P., Onautshu, O., Mpiana, P. T., & Haesaert, G. (2020). Antifungal Activity on the Strain of Lasiodiplodia theobromae and Phytochemical Study of Ageratum conyzoides and Newbouldia laevis from the Kisangani Region / DR Congo. International Journal of Pathogen Research, 5(4), 1-10. https://doi.org/10.9734/ijpr/2020/v5i430138
Section
Original Research Article

References

Mbenoun M, Momo ZEH, Samuels G, Nsouga AF, Nyasse S. Dieback due to Lasiodiplodia theobromae, a new constraint to cocoa production in Cameroon. Plant Pathology. 2008;57:381. DOI: 10.1111/j.1365-3059.2007.01755.x

Punithalingam E. Botryodiplodia theobromae, CMI descriptions of pathogenic fungi and bacteria. No.519. Commonwealth Mycological Institute. Kew, Surrey, England; 1976.

Khanzada MA, Lodhi AM, Shahzad S. Mango dieback and gummosis in Sindh Pakistan caused by Lasiodiplodia theobromae. Plant Health Progress; 2004. Available:http://www.plantmanagementnetwork.org

Ko WH, Wang IT, Ann PJ. Lasiodiplodia theobromae as a causal agent of kumquat dieback in Taiwan. Plant Disease. 2004;88:1383.

Kannan C, Karthik M, Priya K. Lasiodiplodia theobromae causes a damaging dieback of cocoa in India. Plant Pathol. 2010;59:410

Dionisio GA, Frances LMG. Lasiodiplodia theobromae causes vascular streak dieback (VSD)–like symptoms of cacao in Davao Region, Philippines. Australasian Plant Dis. Notes. 2017;12:54. DOI 10.1007/s13314-017-0279-9

Kwembe JTK, Mbula JP, Onautshu O, Mpiana PT, Haesaert G. Evaluation in vitro d’activité antifongique d’Aloe vera, de Moringa oleifera et Newbouldia laevis sur la souche de Lasiodiplodia theobromae dans la Région de la Kisangani/RDCONGO. ISSN 2412-9771 (Print) ISSN 2412-897X (Online). Sch Bull. 2020;6(5):111-118.

De Jaeger C, Voronska E, Fraoucene N, Cherin P. Exposition chronique aux pesticides, Rôle de notre alimentation. Revue "Médecine et Longévité. Institut de médecine et physiologie de la longévité – IDJ – PARIS, Version du 22 Janvier 2018 Available:www.institutdejager.com

Adjanohou JE. Contribution au recensement des plantes médicinales de Côte-d’Ivoire. CRES, Université CI, Centre national de floristique. 1979;358.

Tra BI FH, Guy M Irié, Kohué CC N’gaman & Clejesson HBM. Études de quelques plantes thérapeutiques utilisées dans le traitementde l’hypertension artérielle et du diabète : deux maladies émergentes enCôte d’Ivoire. Sciences & Nature. 2008;5(1):39-48

N’Guessan K, Kouassi Konan E, Tiébré MS. Plantes utilisées dans le traitenement des troubles gynéco-obstétriques par les peuples Abbey et Krobou d’Agboville (Côt-d’Ivoire). Phytothérapie clinique. 2009;7:262–274. DOI 10.1007/s10298-009-0411-x

Kwembe JTK, Asumani MK, Onautshu O, Mpiana PT, Haesaert G. In vitro evaluation of antifungal activity of Ageratum conyzoides, Basella alba and Mitracarpus villosus on the strain of Lasiodiplodia theobromae in the Kisangani Region / DRCongo. Tropicultura (sous presse); 2020.

Fontem LA, Chikoye D, Fokunang C, Ndifon EM. Weeds as potential biopesticides in Taro leaf blight disease management. Research Application Summary. 2014;313–316

Minaflinou SSIY, Virgile E, Azando B, Olounlade PA, Hounzangbe-Adote MA. Effets combinés des feuilles de Newbouldia laeviset de Zanthoxylumzanthoxyloïdes sur les nématodes parasites gastro-intestinaux des ovins Djallonké. Int. J. Biol. Chem. Sci. 2090;9(4):2078-2090, ISSN 1997-342X (Online). 2015; ISSN 1991-8631. Available: http://www.ifg-dg.org

Azando EVB, Olounladé AP, Hounzangbé– AMS, Hoste H. Effets anthelminthiques in vivo de la poudre de feuilles de Zanthoxylumzanthoxyloïdes et de Newbouldia laevis sur les nématodes parasites gastro-intestinaux des chevreaux Djallonké. Int. J. Biol. Chem. Sci. 2011;5(3):1054-1062. DOI: http://dx.doi.org/10.4314/ijbcs.v5i3.7220 8

Spotts RA & Cervantes LA. Populations, pathogenicity, and benomyl resistance of Botrytis spp., Penicillium spp., and Mucor piriformis in packinghouses. Plant Disease. 1986;70:106-108.

Avenot H, Morgan DP & Michailides TJ. Resistance to pyraclostrobin, boscalid and multiple resistance to pristine (pyraclostrobin + boscalid) fungicide in Alternaria alternata causing Alternaria late blight of pistachios in California. Plant Pathology. 2008;57(1):135–140.

Kwembe JTK, Onautshu DO, Mpiana PT, Bekaert B, Haesaert G. Antifungal activity on Lasiodiplodia theobromae and phytochemical study of Mitracarpus villosus and Moringa oleifera from Kisangani (D.R.CONGO).European Journal of Pharmaceuticaland Medical Research, ISSN 2394-3211 EJPMR. 2020;7(10):125-133

Nshimba S. Etude floristique, écologique et phytosociologique des forêts de l'ile Mbiye à Kisangani, R.D.Congo. Thèse de doctorat in edite, ULB. 2008;255.

Monusco, Ville et Population de la RD Congo, [Online] ; 2003. Available:http://monusco.unmissions.org/Default.aspx?tabid=11204& (juin ,2015)

B; Pharmacognosie, Phytochimie, Plantes médicinales, (3ème éd.).Lavoisier Techniques & Documentation. Paris. 1999;369-388.

Bidie ADP, N’guessan BB, Yapo AF, N’guessan JD, Djaman AJ. Activités antioxydantes de dix plantes médicinales de la pharmacopée ivoirienne. Sciences & Nature. 2011;8(1):1-11.

Yapi AB, Camara D, Coulibaly K, Zirihi GN. Étude botanique, tri phytochimique et évaluation de l’activité antifongique de l’extrait éthanolique des feuilles de Ecliptaprostrata (L.) L. (Asteraceae) sur la croissance in vitro de trois souches fongiques. Journal of Applied Biosciences. 2018;125:12581-12589. ISSN 1997-5902

Eyang EM. Etude de la phytochimique et des activités antibactériennes et antifongiques de cinq plantes médicinales utilisées dans le traitement traditionnel des dermatoses au Mali. Thèse de doctorat en Sciences pharmaceutiques, Université de Bamako. 2007;70

Bokota M. Etude de l’activité antifalcémiante et de la stabilité physico-chimique des anthocyanes de quelques espèces des genres Justicia, Ficus etAnnona récoltées dans les Districts de la Tshopo et de l’Ituri en RD Congo. Thèse de doctorat en Sciences chimiques, inedite, Université de Kisangani. 2012;63.

Bruneton J. Pharmacognosie, Phytochimie, plantes médicinales. Lavoisier. 2009;4éd: 1292.

Chung KT. Tannins and human health: a review. Critical reviews food science and nutrition. 1998;38(6):421-64.

Timite G. Isolement et caractérisation des saponosides de plantes de la famille des Alliaceae, caryophyllaceae et Polygalaceae et évaluation de leurs activités cytot-oxiques sur cellules tumorales. Thèse de doctorat en Sciences pharmaceutiques, Université de Bourgogne, Ecole Doctorale. 2012;67.

Bouazza F, Hassikou R. Activité antifongique in vitro de la pulpe foliaire d’Aloe vera. Bull. Soc. Pharm. Bordeaux. 2011;150(1-4):95-106.

Snyder L – Kirkl J. Introduction to modern liquid chromatography. Ed. Wiley New york. 1979;81.

Bagre I, Bahi C, Ouattara K, Zirihi GN, Djaman AJ, Coulibaly A. Étude botanique et exploration de l’activité antifongique de Morinda morindoides (Baker) Milne-Redh. sur la croissance in vitro de Cryptococcus neoformans. Pharmacognosie. Phyto-thérapie. Springer-Verlag France. 2011;9:136–141. DOI 10.1007/s10298-011-0612-y

Saraka AI, Abo K, Ouattara KE, Zirihi GN. Étude botanique, tri phytochimique et évaluation in vitro de l’activité antifongique des extraits de feuilles de Mallotusoppositifolius (Geisel.) Müll. Arg (Euphorbiaceae) sur Fusarium sp. et Phytophthora sp. deux champignons phytopathogènes. Journal of Animal & Plant Sciences (J.Anim.PlantSci. ISSN 2071-7024). 2019;41(2):6903-6915.

Sinha SKP, Dogra JV. A survey of plants bhagalpur and santhal pargana for saponins, flavonoïds and alkaloids. 1985;(2):77-86.

Hui WH, Lee KL. Triterpenoïd and steroid constituents of some lactue and Ageratum species of Hong-Kong phytochemistry. 1987;10(4):899-901

Kuete V, Tankeo SB, Saeed ME, Wiench B, Tane P, Efferth T. Cytotoxicity and modes of action of five Cameroonian medicinal plants againstmulti-factorial drug resistance of tumor cells. J Ethnopharmacol. 2014;153(1):207–19.

Dandjesso C, Klotoé J, Dougnon T, Sègbo J, Atègbo J, Gbaguidi F, et al. Phytochemistry and hemostatic properties of some medicinal plants sold as anti-hemorrhagic in Cotonou markets (Benin). Indian J Sci Technol. 2012;5(8):3105–9.

Usman H, Osuji JC. Phytochemical and in vitro antimicrobial assay of the leaf extract of Newbouldia laevis. Afr J TraditComplementAltern Med. 2007;4(4):476–80.

Kasmi M, Aourach M, El Boukari M, Barrijal S, Essalmani H. Efficacité des extraits aqueux des plantes aromatiques et médicinales contre la pourriture grise de la tomate au Maroc. Comptes Rendus Biologies. C. R. Biologies. 2017;340:386–393

Gruiz K, Biacs PA. Membrane lipid composition of Trichoderma strains and their sensitivity to saponin and polyene antibiotics, in: P.A. Biacs, K. Gruiz, T. Kremmer (Eds.), Biological role of plant lipids, Plenum Press, New York, Londres. 1989;417–420.

Béné K, Camara D, Fofie NBY, Kanga Y, Yapi AB, Yapo YC, Ambe SA et Zirihi GN. Étude ethnobotanique des plantes médicinales utilisées dans le Département de Transua, District du Zanzan (Côte d’Ivoire). Journal of Animal &Plant Sciences. 2016;27(2):4230-4250

Komlaga CG, Agyare R, Akosua D, Kwao Mensah ML, Annan K, Loiseau PM, Champy P. Medicinal plants and finished marketed herbal products used in the treatment of malaria in the Ashanti region, Ghana. Journal of Ethnopharmacology. 2015;172(2015)333–346