In vitro, In vivo and In situ, Effect of Mancozeb 80 WP on Colletotrichum gloeosporioides (Penz.) Penz. and Sacc., Causative Agent of Anthracnose of Cashew (Anacardium occidentale L.) in Chad and Cameroon
-
Ngoh Dooh Jules Patrice
- Deurnaye Placide
- Abdoul Madjerembe
- Mbou T. Pavel Rony
- Djongnang Gabriel
- Boydoul F. Ulrich
- Djile Bouba
- Ambang Zachee
International Journal of Pathogen Research,
Page 1-14
DOI:
10.9734/ijpr/2021/v6i330161
Abstract
Aims: The aim of this work was to evaluate effect of Mancozeb 80 WP against Colletotrichum gloeosporioides, the agent responsible of anthracnose of cashew tree (Anacardium occidentale L.).
Study Design: The experimental design was in completely randomized blocks with three replications.
Place and Duration of Study: in vitro and in vivo experiments were performed in Laboratory of Department of Biological Sciences, University of Maroua during six months. Field trial was carried out in Kelo, Chad during three months.
Methodology: Isolates were obtained from diseased organs (leaves and fruits) from Kélo in Chad and Maroua in Cameroon. The concentrations used in the laboratory were C1 (5 mg/ml), C2 (0.5 mg/ml), C3 (0.05 mg/ml), C4 (0.005 mg/ml), C5 (0.0 mg/ml). Radial growth, sporulation, conidial germination and pathogenicity were used to characterize and evaluate the effect of Mancozeb on the isolates in vitro. The preventive test was performed on three-month-old plants previously treated with Mancozeb. The concentration of 5 g/l was applied to the field and the incidence and severity were used to calculate the AUIPC (Area Under Disease Incidence Progress Curve) and AUSiPC (Area Under Disease Severity Index Progress Curve) curves.
Results: Mancozeb reduced radial growth of all isolates at concentrations C1 (5 mg/ml), C2 (0.5 mg/ml) and C3 (0.05 mg/ml). The percentages of inhibition ranged from 50 to 100%. Mancozeb 80 WP completely (100%) inhibited the germination of C. gloeosporioides conidia in vitro. Mancozeb has protected cashew plants in vivo at the concentration C1 (5 mg/ml). AUIPC and AUSiPC were higher on control plants and lower on Mancozeb-treated plants.
Conclusion: Mancozeb 80 WP may be associated in integrated pest management strategy against anthracnose.
Keywords:
- Anacardium occidentale L.
- Colletotrichum gloeosporioides
- anthracnose
- Mancozeb 80 WP
- inhibition
- AUIPC
- AUSiPC
- mancozeb
- Anacardium occidentale L
- pathogen.
How to Cite
Patrice, N. D. J., Placide, D., Madjerembe, A., Rony, M. T. P., Gabriel, D., Ulrich, B. F., Bouba, D., & Zachee, A. (2021). In vitro, In vivo and In situ, Effect of Mancozeb 80 WP on Colletotrichum gloeosporioides (Penz.) Penz. and Sacc., Causative Agent of Anthracnose of Cashew (Anacardium occidentale L.) in Chad and Cameroon. International Journal of Pathogen Research, 6(3), 1-14. https://doi.org/10.9734/ijpr/2021/v6i330161
References
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FAO. La situation mondale de alimentation et de agriculture. 2015;202.
De figueiredo RW, Lajolo FM, Alves RE, Filgueiras HAC. Phsysical–chemical changes in early dwarf cashew pseudo fruits during development and maturation. Food Chemistry. 2001;77:343–347.
Hamawa Y, Dona A, Kanmegne ON, Mbaye C, Niwah Awono JMDK, Mapongmetsem PM. Effet du poids de noix et de la dose d’engrais sur la germination et la croissance de l’anacardier (Anacardium occidentale L., Anacardiaceae) dans la savane guinéenne du Cameroun. Afrique Science. 2019;15(5): 302–312
Adeigbe OO, Olasupo FO, Adewale BD, Muyiwa AA. A review of cashew research and production in Nigeria in the last four decades, Science Research Essays. 2015;10:196-209.
Kouassi EKA. Contribution à la valorisation des sous-produits agricoles en bioproduits, Thèse De Doctorat De l’Université De Toulouse. 2018;9.
Dedehou ESCA, Dossou J, Soumanou MM. Etude diagnostique des technologies de transformation de la pomme de cajou en jus au Bénin. International Journal of Biological and Chemical Sciences. 2015;9(1):371–387.
Madou C, Sali B, Awe DV, Nome A. Evaluation of cashew (Anacardium occidentale) accessions diversity of in large pools of production in Cameroon and their germ performance to increase productivity. Journal of Animal & Plant Sciences. 2020;46(2):8212-8225.
Available:https://doi.org/10.35759/JAnmPlSci.v46-2.5
Sali B, Madou C, Nome A, Kuate J. Caractérisation socio-économique des grands bassins de productionsd’anacardiers (Anacardium occidentale) et étude comportementale de leur peuplement dans le Cameroun septentrional. Int. J. Biol. Chem. Sci. 2020; 14(6):2094-2111.
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Afouda LCA, Zinsou V, Balogoun RK, Onzo A, Ahohuendo BC. Inventaire des agents pathogènes de l’anacardier (Anacardium occidentale L.) au Bénin, Bulletin de la Recherche Agronomique du Bénin (BRAB). 2013;73:13-19.
Delgado.CG, Guy C. Principaux insectes nuisibles à l’anacardier en Amazonie péruvienne : Données préliminaires. Fruits. 2014;69(4):293–302.*
Kone D, Abo K, Ouali NM, Cherif M, N’depo OR, Soro S. Maladies et insectes ravageurs de l'anacardier. Biological Control of Plant Pathology. 2015;1:14.
Wonni I, Sereme D, Ouedraogo I, Kassankagno AI, Dao I, Ouedraogo L, et al. Diseases of cashew nut plant in Burkina Faso. Advances in Plants & Agric. Resea. 2017;6:1-8
Freire FCO, Cardoso JE, Dos Santos AA, Viana FMP. Diseases of cashew nut plants (Anacardium occidentale L.) in Brazil. Crop Protection. 2002;21:489–49.
Sarkar AK. Anthracnose diseases of some common medicinally important fruit plants. Journal of Medicinal Plants Studies. 2016;4(3): 233-236.
Haider M, Bukhari SAA, Binyamin R, Habib A. Fungi associated with guava anthracnose and management of Colletotrichum gloeosporioides through biological and chemical means. Pak. J. Phytopathol. 2016; 28(02):153-160.
Silué N, Abo K, Camara B, Soro S, Ouattara GM, Tuo S, et al. Effect of some synthetic fungicides on the in vitro growth of Colletotrichum gloeosporioides, Causative agent of cashew tree anthracnose in Côte d’Ivoire. Asian J. Crop Sci. 2017;9:149-158.
Ngoh Dooh JP, Heu A, Kone NAN, Mboussi SB, Asta DBC, Tchoupou TDB, et al. Dieback of cashew (Anacardium occidentale) in Cameroon. Pak. J. Phytopathol. 2020;32(02):231-239.
DOI: 10.33866/phytopathol.030.02.0605
Khatoon A, Mohapatra A, Kunja Bihari S. Major diseases of cashew (Anacardium Occidentale L.) Caused by fungi and their control in Odisha, India. International Journal of Biosciences. 2017;11(1):68-74
Filoda G. Impact of some fungicides on mycelium growth of Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. Pesticides. 2008;3-4:109-116.
Tonon D, Sikirou R, Adomou AC, Zinsou V, Zocli B, N’Djolosse K, et al. Efficacité des fongicides Mancozèbe 80 WP et Chlorothalonil-Carbendazime 65 SC contre Colletotrichum gloeosporioides agent causal de l’anthracnose de l’anacardier au Bénin. Int. J. Biol. Chem. Sci. 2017;11(5):2093-2105.
DOI: https://dx.doi.org/10.4314/ijbcs.v11i5.13
Silué N, Abo K, Camara B, Soro S, Ouattara GM, Tuo S, et al. Effect of some Synthetic Fungicides on the in vitro Growth of Colletotrichum gloeosporioides, Causative agent of cashew tree anthracnose in Côte d’Ivoire. Asian J. Crop Sci. 2018;9:49–158
Asta DBC. Identification des maladies de l’anacardier (Anacarsium occidentale L.) dans l’Extrême Nord Cameroun (Maroua et Yagoua). Memoire de Master II. en Biotechnologies Végétales. Université de Maroua, 2019;80.
Barnett HL, Hunter BB. Illustrated genera of imperfecti fungi, 4th edition MacMilan. 1977;21.
Singh SR, Jackai LEN, Thottappilly G, Cardewell KF, Myers GO. Status of research on constraints to cowpea production, In Biotechnology: enchancing research on tropical crops in Africa éd, 1993;21-26.
Nyassé S, Cilas C, Hérail C. Blaha G. leaf inoculation as an early screening test of cocoa (Theobroma cacao L.) resistance to Phytophthora black pod disease: Crop protection. 1995;14:657-663
Yang YL, Liu ZY, Cai L, Hyde KD, Yu ZN, McKenzie EHC. Colletotrichum species of anthracnose of Amaryllidaceae. Fungal Diversity. 2015;39:123–146 .
Widmer TL, Laurent N. Plant extracts containing caffeic acid and rosmarinic acid inhibit zoospore germination of Phytophthora spp pathogenic to Theobroma cacao. European Journal of Plant Pathology. 2006;115:377-388.
Cooke BM. Disease assessment and yield loss. In: Cooke BM, Jones DG, Kaye B. (eds), Epidemiol. plant Dis. Springer, Netherlands. 2006;43-80.
Jeger Mj and Viljanen-Rollinson SH. The use of the area under the disease progress curve to assess quantitative disease resistance in crop cultivars, Theor Appl Genet, 2001; 102; 32-40.
Muengula Manyi M, Mukwa L, Nkongolo KK, Tshilenge Djim P, Winter S, Bragard C, et al. Assessing reactions of genetically improved and local cassava varieties to cassava mosaic disease (CMD) infection in a savannah region of the DR-Congo. American Journal of Plant Sciences. 2013;4(4):824-837.
DOI: 10.4236/ajps.2013.44101.
Sanders GM, Korsten L. A comparative morphological study of South African avocado and mange isolates of Colletotrichum gloeosporioides. Canadian Journal of Botany. 2003;81:877-885.
Serra lMRS, Menezes M, Coelho RSB, Ferraz GDMG, Montrroyos AVV, Martins LSS. Morphophysiological and molecular analysis in the differentiation of Colletotrichum gloeosporioides isolats from cashew and mange trees. Anais da Academia Pernambucana de Ciência Agronômica. 2006;3:216-241
Randriantsalama AR, Randrianaivoarivony JM, Ramalanjaona VL. L’utilisation de la lutte chimique et de la résistance variétale contre le mildiou de la pomme de terre à Madagascar, Afr Crop Sci J. 2014;22:959–968.
Hamdache A, Lamarti A, Badoc A. Résistance in vitro de Botrytis cinerea à trois fongicides, Bull Soc Pharm Bordeaux. 2010;149:103-114.
Mbaye N, Diedhiou PM, Ndiaye S, Samb PI. [In vitro biological characterization of Colletotrichum gloeosporioides Penz, agent of anthracnose of mango (Mangifera indica L.)]. J. Sci. Eng. 2008;9:21-27. (In French).
Ngoh Dooh JP, Ambang Z, TihEwola A, Heu A, Kosma P, Maho Yalen EJ, et al. Screening and the effect of extracts of Thevetia peruviana on the development of Colletotrichum gloeosporioides, causal agent of cassava anthracnose disease. E3 Journal of Agricultural Research and Development. 2014;4(4):054-065
Moses E, Akrofi S, Beseh P. Anthracnose of cashew; 2016.
Available:http://www.plantwise.org/FullTextPDF/2017/ 20177800626.pdf
Chacko SP, Gokulapalan C. In vitro study of fungicides and biocontrol agents against Colletotrichum capsici causing anthracnose of chilli (Capsicum annuum L.). Int. J. Applied Pure Sci. Agric. 2014;1:93-98
Haider M, Bukhari SAA, Binyamin R, Habib A. Fungi associated with guava anthracnose and management of Colletotrichum gloeosporioides through biological and chemical means. Pak. J. Phytopathol. 2016;28(02):153-160.
Duamkhanmanee R. Natural essential oils from lemon grass (Cymbopogon citratus) to control postharvest anthracnose of mango fruit, Int. J. Biotechnol. 2008;10:104-108 .
Chand G, Srivastava JN, Kumar S, Kumar S. Efficacy of different fungicide against anthracnose of mango (Mangifera indica L.) in Eastern Bihar. Internat. J. Agric. Sci. 2013;9:204–206.
Cole JT, Cole JC, Conway KE. Effectiveness of selected fongicides applied with or without surfactant in controlling anthracnose on three cultivars of Euonymus fortunei, Journal of Applied Horticulture, 2005;7:16-19.
Nyabyenda P. Les plantes cultivées en régions tropicales d'altitude d'Afrique. T.1. Légumineuses alimentaires. Plantes à tubercules et racines. Céréales. Les presses Universitaires de Liège. 2005;225.
Sikirou R, Bello S, Zocli B, Adéfadjo A. Efficacité du fongicide COGA 80 WP (Mancozèbe 800 g/kg) contre la cercosporiose de la laitue et le chancre de l’amarante. Rapport d’activité, LDC/INRAB, Bénin, 2012;11.
FAO. La situation mondale de alimentation et de agriculture. 2015;202.
De figueiredo RW, Lajolo FM, Alves RE, Filgueiras HAC. Phsysical–chemical changes in early dwarf cashew pseudo fruits during development and maturation. Food Chemistry. 2001;77:343–347.
Hamawa Y, Dona A, Kanmegne ON, Mbaye C, Niwah Awono JMDK, Mapongmetsem PM. Effet du poids de noix et de la dose d’engrais sur la germination et la croissance de l’anacardier (Anacardium occidentale L., Anacardiaceae) dans la savane guinéenne du Cameroun. Afrique Science. 2019;15(5): 302–312
Adeigbe OO, Olasupo FO, Adewale BD, Muyiwa AA. A review of cashew research and production in Nigeria in the last four decades, Science Research Essays. 2015;10:196-209.
Kouassi EKA. Contribution à la valorisation des sous-produits agricoles en bioproduits, Thèse De Doctorat De l’Université De Toulouse. 2018;9.
Dedehou ESCA, Dossou J, Soumanou MM. Etude diagnostique des technologies de transformation de la pomme de cajou en jus au Bénin. International Journal of Biological and Chemical Sciences. 2015;9(1):371–387.
Madou C, Sali B, Awe DV, Nome A. Evaluation of cashew (Anacardium occidentale) accessions diversity of in large pools of production in Cameroon and their germ performance to increase productivity. Journal of Animal & Plant Sciences. 2020;46(2):8212-8225.
Available:https://doi.org/10.35759/JAnmPlSci.v46-2.5
Sali B, Madou C, Nome A, Kuate J. Caractérisation socio-économique des grands bassins de productionsd’anacardiers (Anacardium occidentale) et étude comportementale de leur peuplement dans le Cameroun septentrional. Int. J. Biol. Chem. Sci. 2020; 14(6):2094-2111.
DOI: https://dx.doi.org/10.4314/ijbcs.v14i6.13.
Afouda LCA, Zinsou V, Balogoun RK, Onzo A, Ahohuendo BC. Inventaire des agents pathogènes de l’anacardier (Anacardium occidentale L.) au Bénin, Bulletin de la Recherche Agronomique du Bénin (BRAB). 2013;73:13-19.
Delgado.CG, Guy C. Principaux insectes nuisibles à l’anacardier en Amazonie péruvienne : Données préliminaires. Fruits. 2014;69(4):293–302.*
Kone D, Abo K, Ouali NM, Cherif M, N’depo OR, Soro S. Maladies et insectes ravageurs de l'anacardier. Biological Control of Plant Pathology. 2015;1:14.
Wonni I, Sereme D, Ouedraogo I, Kassankagno AI, Dao I, Ouedraogo L, et al. Diseases of cashew nut plant in Burkina Faso. Advances in Plants & Agric. Resea. 2017;6:1-8
Freire FCO, Cardoso JE, Dos Santos AA, Viana FMP. Diseases of cashew nut plants (Anacardium occidentale L.) in Brazil. Crop Protection. 2002;21:489–49.
Sarkar AK. Anthracnose diseases of some common medicinally important fruit plants. Journal of Medicinal Plants Studies. 2016;4(3): 233-236.
Haider M, Bukhari SAA, Binyamin R, Habib A. Fungi associated with guava anthracnose and management of Colletotrichum gloeosporioides through biological and chemical means. Pak. J. Phytopathol. 2016; 28(02):153-160.
Silué N, Abo K, Camara B, Soro S, Ouattara GM, Tuo S, et al. Effect of some synthetic fungicides on the in vitro growth of Colletotrichum gloeosporioides, Causative agent of cashew tree anthracnose in Côte d’Ivoire. Asian J. Crop Sci. 2017;9:149-158.
Ngoh Dooh JP, Heu A, Kone NAN, Mboussi SB, Asta DBC, Tchoupou TDB, et al. Dieback of cashew (Anacardium occidentale) in Cameroon. Pak. J. Phytopathol. 2020;32(02):231-239.
DOI: 10.33866/phytopathol.030.02.0605
Khatoon A, Mohapatra A, Kunja Bihari S. Major diseases of cashew (Anacardium Occidentale L.) Caused by fungi and their control in Odisha, India. International Journal of Biosciences. 2017;11(1):68-74
Filoda G. Impact of some fungicides on mycelium growth of Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. Pesticides. 2008;3-4:109-116.
Tonon D, Sikirou R, Adomou AC, Zinsou V, Zocli B, N’Djolosse K, et al. Efficacité des fongicides Mancozèbe 80 WP et Chlorothalonil-Carbendazime 65 SC contre Colletotrichum gloeosporioides agent causal de l’anthracnose de l’anacardier au Bénin. Int. J. Biol. Chem. Sci. 2017;11(5):2093-2105.
DOI: https://dx.doi.org/10.4314/ijbcs.v11i5.13
Silué N, Abo K, Camara B, Soro S, Ouattara GM, Tuo S, et al. Effect of some Synthetic Fungicides on the in vitro Growth of Colletotrichum gloeosporioides, Causative agent of cashew tree anthracnose in Côte d’Ivoire. Asian J. Crop Sci. 2018;9:49–158
Asta DBC. Identification des maladies de l’anacardier (Anacarsium occidentale L.) dans l’Extrême Nord Cameroun (Maroua et Yagoua). Memoire de Master II. en Biotechnologies Végétales. Université de Maroua, 2019;80.
Barnett HL, Hunter BB. Illustrated genera of imperfecti fungi, 4th edition MacMilan. 1977;21.
Singh SR, Jackai LEN, Thottappilly G, Cardewell KF, Myers GO. Status of research on constraints to cowpea production, In Biotechnology: enchancing research on tropical crops in Africa éd, 1993;21-26.
Nyassé S, Cilas C, Hérail C. Blaha G. leaf inoculation as an early screening test of cocoa (Theobroma cacao L.) resistance to Phytophthora black pod disease: Crop protection. 1995;14:657-663
Yang YL, Liu ZY, Cai L, Hyde KD, Yu ZN, McKenzie EHC. Colletotrichum species of anthracnose of Amaryllidaceae. Fungal Diversity. 2015;39:123–146 .
Widmer TL, Laurent N. Plant extracts containing caffeic acid and rosmarinic acid inhibit zoospore germination of Phytophthora spp pathogenic to Theobroma cacao. European Journal of Plant Pathology. 2006;115:377-388.
Cooke BM. Disease assessment and yield loss. In: Cooke BM, Jones DG, Kaye B. (eds), Epidemiol. plant Dis. Springer, Netherlands. 2006;43-80.
Jeger Mj and Viljanen-Rollinson SH. The use of the area under the disease progress curve to assess quantitative disease resistance in crop cultivars, Theor Appl Genet, 2001; 102; 32-40.
Muengula Manyi M, Mukwa L, Nkongolo KK, Tshilenge Djim P, Winter S, Bragard C, et al. Assessing reactions of genetically improved and local cassava varieties to cassava mosaic disease (CMD) infection in a savannah region of the DR-Congo. American Journal of Plant Sciences. 2013;4(4):824-837.
DOI: 10.4236/ajps.2013.44101.
Sanders GM, Korsten L. A comparative morphological study of South African avocado and mange isolates of Colletotrichum gloeosporioides. Canadian Journal of Botany. 2003;81:877-885.
Serra lMRS, Menezes M, Coelho RSB, Ferraz GDMG, Montrroyos AVV, Martins LSS. Morphophysiological and molecular analysis in the differentiation of Colletotrichum gloeosporioides isolats from cashew and mange trees. Anais da Academia Pernambucana de Ciência Agronômica. 2006;3:216-241
Randriantsalama AR, Randrianaivoarivony JM, Ramalanjaona VL. L’utilisation de la lutte chimique et de la résistance variétale contre le mildiou de la pomme de terre à Madagascar, Afr Crop Sci J. 2014;22:959–968.
Hamdache A, Lamarti A, Badoc A. Résistance in vitro de Botrytis cinerea à trois fongicides, Bull Soc Pharm Bordeaux. 2010;149:103-114.
Mbaye N, Diedhiou PM, Ndiaye S, Samb PI. [In vitro biological characterization of Colletotrichum gloeosporioides Penz, agent of anthracnose of mango (Mangifera indica L.)]. J. Sci. Eng. 2008;9:21-27. (In French).
Ngoh Dooh JP, Ambang Z, TihEwola A, Heu A, Kosma P, Maho Yalen EJ, et al. Screening and the effect of extracts of Thevetia peruviana on the development of Colletotrichum gloeosporioides, causal agent of cassava anthracnose disease. E3 Journal of Agricultural Research and Development. 2014;4(4):054-065
Moses E, Akrofi S, Beseh P. Anthracnose of cashew; 2016.
Available:http://www.plantwise.org/FullTextPDF/2017/ 20177800626.pdf
Chacko SP, Gokulapalan C. In vitro study of fungicides and biocontrol agents against Colletotrichum capsici causing anthracnose of chilli (Capsicum annuum L.). Int. J. Applied Pure Sci. Agric. 2014;1:93-98
Haider M, Bukhari SAA, Binyamin R, Habib A. Fungi associated with guava anthracnose and management of Colletotrichum gloeosporioides through biological and chemical means. Pak. J. Phytopathol. 2016;28(02):153-160.
Duamkhanmanee R. Natural essential oils from lemon grass (Cymbopogon citratus) to control postharvest anthracnose of mango fruit, Int. J. Biotechnol. 2008;10:104-108 .
Chand G, Srivastava JN, Kumar S, Kumar S. Efficacy of different fungicide against anthracnose of mango (Mangifera indica L.) in Eastern Bihar. Internat. J. Agric. Sci. 2013;9:204–206.
Cole JT, Cole JC, Conway KE. Effectiveness of selected fongicides applied with or without surfactant in controlling anthracnose on three cultivars of Euonymus fortunei, Journal of Applied Horticulture, 2005;7:16-19.
Nyabyenda P. Les plantes cultivées en régions tropicales d'altitude d'Afrique. T.1. Légumineuses alimentaires. Plantes à tubercules et racines. Céréales. Les presses Universitaires de Liège. 2005;225.
Sikirou R, Bello S, Zocli B, Adéfadjo A. Efficacité du fongicide COGA 80 WP (Mancozèbe 800 g/kg) contre la cercosporiose de la laitue et le chancre de l’amarante. Rapport d’activité, LDC/INRAB, Bénin, 2012;11.
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