Antifungal Potential of Struchium sparganophora (Antsbush): A Promising Candidate for Treating Candidiasis and Cryptococcosis
Bibi Ally-Charles *
College of Medical Sciences, University of Guyana, Georgetown, Guyana.
Ede Tyrell
College of Medical Sciences, University of Guyana, Georgetown, Guyana.
Shontel Archer
College of Medical Sciences, University of Guyana, Georgetown, Guyana.
Vishmeta Seenarine
College of Medical Sciences, University of Guyana, Georgetown, Guyana.
Tashanna Gomes
College of Medical Sciences, University of Guyana, Georgetown, Guyana.
Joel Semple
College of Medical Sciences, University of Guyana, Georgetown, Guyana.
Basil Dey
Faculty of Natural Sciences, University of Guyana, Georgetown, Guyana.
Andrew Boyle
Eureka Medical Laboratory, Georgetown, Guyana.
Andrew Hutson
College of Medical Sciences, University of Guyana, Georgetown, Guyana.
Obena Vanlewin
College of Medical Sciences, University of Guyana, Georgetown, Guyana.
Karishma Jeeboo
College of Medical Sciences, University of Guyana, Georgetown, Guyana.
*Author to whom correspondence should be addressed.
Abstract
Aims: The incidence of Candidiasis and Cryptococcosis, and the emergence of acquired antifungal resistance have increased rapidly. This study aimed to evaluate the effectiveness of S. sparganophora leaf extracts against C. albicans and C. neoformans; and to determine whether there was any difference between the antifungal effects of S. sparganophora leaf extracts and two conventional antifungal agents (AFAs).
Study Design: Experiment-based study.
Place and Duration of Study: S. sparganophora leaves was obtained from a site in Kimbia, Berbice River, Guyana; identified by the Biodiversity Centre and tested at the Main Laboratory at the College of Medical Sciences, University of Guyana during January- August 2023.
Methodology: Dried pulverised leaves were macerated using different solvents and concentrated using a rotary evaporator. Sterile filter paper discs were soaked in different concentrations of the various extracts. Antifungal discs were placed on Sabouraud’s Dextrose Agar plates seeded with fungi. All plates were incubated and zones of inhibitions (ZOIs) were measured and expressed as mean ± SD.
Results: S. sparganophora leaf extracts showed large ZOI especially at the 100 mg/ml concentrations against all organisms tested. The largest ZOI was seen for the hexane extract against C. neoformans (35.5±5 mm) and C. Albicans In-House B (18.5±3.5 mm) at 100 mg/ml concentrations. No ZOI was observed against the AFA fluconazole by C. albicans In-House B, while large ZOI were observed with the hexane and ethyl acetate extracts at 100 mg/ml concentrations. Statistical analysis showed correlation between the ZOI and concentrations, ZOI and solvent extract, and ZOI and type of fungi.
Conclusion: In conclusion, S. sparganophora leaf extracts have great antifungal activity and in some cases showed greater activity when compared to conventional AFAs.
Keywords: Antifungal agents, S. sparganophora, Candidiasis, Cryptococcosis, Solvents, Inhibition zones