Salmonella enterica Encoding blaNDM Isolated at Saint-Camille Hospital in Ouagadougou, Burkina Faso
Lionel Eliada Benoît BAMBARA
*
Laboratory of Molecular Biology and Genetics (LAMBIOGEN), Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Amana METUOR DABIRE
Daniel Ouezzin Coulibaly University, BP 176 Dedougou, Burkina Faso.
Nicolas OUEDRAOGO
National Institute of Public Health, Malaria Research and Training Center, Ouagadougou, Burkina Faso.
Damis Yves Patrik BOUNIOUNOU
Laboratory of Molecular Biology and Genetics (LAMBIOGEN), Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Olawoumi Fabrice KOUTA
Laboratory of Molecular Biology and Genetics (LAMBIOGEN), Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Rabietou NIKIEMA
Laboratory of Molecular Biology and Genetics (LAMBIOGEN), Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Olivia Rhaïna BADINI
Laboratory of Molecular Biology and Genetics (LAMBIOGEN), Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou 03, Burkina Faso.
Jacques SIMPORE
Annigoni Biomolecular Research Center (ABRCE), 01 BP 364 Ouagadougou 01, Burkina Faso.
*Author to whom correspondence should be addressed.
Abstract
Introduction: Salmonella enterica is a zoonotic pathogen of concern to human and animal health worldwide. Salmonellosis is responsible for significant morbidity and mortality and has a considerable global socioeconomic impact. With the aim of eradicating Salmonellosis caused by Salmonella enterica, we aimed to investigate multidrug resistance in NDM-type carbapenemase- producing S. enterica strains in patients received at HOSCO.
Methodology: In the study, 278 Gram-negative bacilli were collected, including 18 strains of S. enterica. The DNA of the antibiotic-resistant strains was analyzed by conventional PCR for gene search blaNDM using specific primers.
Results: The age group most affected by salmonellosis is 20 to 40 years. Thus, we observed a high resistance of 83% to Amoxiclav, 6% to Imipenem, 22% to Aztreonam, 11% to Meropenem and 17% to Ertapenem. Among the 18 strains of S. enterica 28 % coded for the blaNDM resistance gene. We note that the age group of [20; 40[ years was coded for blaNDM with a frequency of 17%. Also, 11% of S. enterica were multi-resistant.
Conclusion: This study determined the frequency of S. enterica strains producing the NDM resistance enzyme. It also determined the multi-resistance of these strains, the frequency of which is 11%. This calls for increased vigilance in our strategies for combating salmonellosis.
Keywords: Antibiotics, S. enterica, multi-resistant, blaNDM